TBE Buffer

  • Ready-to-use TBE Buffer: save time
  • Guaranteed DNase/RNase free
  • High buffering capacity: no recirculation needed for long runs

TBE Molecular Biology Grade

AccuGENE TBE buffers are prepared with high quality reagents, using ultrapure deionized water of 18 megOhm. Solutions are then filter-sterilized through a 0.45-micron filter.

Advantages and disadvantages of TBE vs TAE in electrophoresis

A key benefit of TBE over TAE is its higher buffering capacity. This allows longer electrophoresis runs without buffer recirculation. In addition, TBE offers the best resolution for fragments smaller than 1500 bp.

Borate is known to be an inhibitor of many enzymatic activities. Therefore, if the separated DNA is to be used in downstream enzymatic reactions, such as cloning or PCR, TAE buffer is recommended instead of TBE.

Buffer composition

  • 0.89 M Tris
  • 0.89 M Boric acid
  • 0.02 M EDTA (disodium salt)
  • pH 8.3

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Lonza website to find your local distributor.
Cat no. Description
LO 50836 AccuGENE 5X TBE Buffer 20L
LO BE08-238Z10 AccuGENE 10X TBE 10 L CUBITAINER
LO BE08-238Z4 AccuGENE 10x TBE 4 L CUBITAINER
LO BE50843 AccuGENE 10X TBE Buffer 1L