SYBR Green Nucleic Acid Gel Stains

  • SYBR Green I stain:
  • Detects as little as 60pg of ds DNA and 1 ng oligonucleotides
  • Optimal for PCR, apoptosis studies, and heteroduplex analysis.
  • SYBR Green II stain:
  • Detects 100 pg of ssDNA and 2 ng of RNA
  • Optimal for RNA electrophoresis and SSCP analysis
SYBR® Green I stain is a highly sensitive fluorescent stain for detecting dsDNA and oligonucleotides in agarose and polyacrylamide gels. Because SYBR® Green's fluorescent signal is greatly enhanced when the dye is bound to nucleic acids, gels stained with SYBR® Green I exhibit an excellent signal-to-noise ratio with no background fluorescence. For maximum performance, gels should be poststained. SYBR® Green I is ideal for detecting small amounts of DNA in low copy/target number PCR products, apoptosis studies, and heteroduplex analysis.

Detection Limits:
-dsDNA at 300nm transillumination: 60 pg
-Oligonucleotides at 300nm transillumination 1-2 ng

SYBR Green II Nucleic Acid Stain:
For ssDNA and RNA, SYBR® Green II offers all the advantages of SYBR® Green I and is ideal for non-isotopic detection of DNA mutations by single-stranded conformation polymorphism (SSCP) analysis. Detection limit at 300 nm ransillumination is 40-80 ng in denaturing gels and 2-4 ng in non-denaturing gels.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Lonza website to find your local distributor.
Cat no. Description
LO 50512 SYBR Green I 2x500ul
LO 50513 SYBR Green I 10x50ul
LO 50522 SYBR Green II 2x500ul
LO 50523 SYBR Green II 10x50ul
LO 50530 SYBR Green Filter 1pcs