Rotaphor 8 Pulsed Field Gel Electrophoresis (PFGE) System

  • Excellent separation of large DNA up to 8 Mb
  • Rotating electrodes for free selection of field angles
  • Application flexibility: PFGE, CHEF, FIGE and ROFE
  • Built-in circulation pump for buffer temperature control
  • Easy to program, easy to run
  • 17 preset programs for different size ranges

PFGE system for separation of large DNA molecules

Pulsed field gel electrophoresis with the Rotaphor 8 system allows the separation of DNA far beyond the 50 kb limit of conventional agarose electrophoresis. Thanks to the patented electrode rotor, the electrical field can be applied in virtually any angle. To achieve a very homogeneous field, the two main rotor electrodes are flanked by 2 sets of secondary electrodes each.

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Buffer temperature control

PFGE gels typically run for many hours, even up to several days. Therefore, the electrophoresis buffer has to be cooled. The Rotaphor electrophoresis chamber has a built-in buffer circulation pump which is connected to an external cooling thermostat. During the run the buffer temperature in the electrophoresis chamber is constantly monitored and precisely controlled by the Rotaphor software.

Easy control

The Rotaphor system includes a PC that controls the electrode rotor and the power supply over the Rotaphor interface card. The Rotaphor software provides 17 pre-set programs for separation of different size ranges. Starting with the pre-set parameters, new applications are quickly optimised. For each pre-set program the software shows a real gel image. Once a custom program has been optimised, a referring gel picture can be uploaded into the software. Thus, the Rotaphor software over time will not only be a control tool, but also a library of successful experiments linked to the underlying separation parameters. By linking programs to combined lists also complex sequences can be programmed.

The Rotaphor System includes:

  • Electrophoresis chamber with electrode rotor and internal buffer circulation
  • power supply
  • gel tray
  • comb (18 teeth)
  • frame for gel casting
  • sample mould
  • computer with interface card and pre-installed Rotaphor software
  • 22" TFT monitor
  • connection cables
  • gel scoop
  • manual

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Analytik Jena website to find your local distributor.
Cat no. Description
AJ 846-021-001 Gel tray (18x13cm) and combs
AJ 846-021-002 Gel tray, 20x20cm
AJ 846-021-003 Set of moulds
AJ 846-021-004 Rotaphor Comb, 25 wells, for 20cm wide gels
AJ 846-021-005 Rotaphor Comb, 40 wells, for 20cm wide gels
AJ 846-021-006 Rotaphor Comb, 50 wells, for 20cm wide gels
AJ 846-021-007 Rotaphor Comb, 18 wells, reduced thickness
AJ 846-021-008 Rotaphor Comb, 5 wells, for 13cm gels
AJ 846-021-009 Rotaphor Comb, 12 wells, for 13cm gels
AJ 846-021-010 Rotaphor Comb, 20 wells, for 13cm gels
AJ 846-021-011 Rotaphor Comb, 18 wells for 20x20cm gels
AJ 846-021-201 Rotaphor ROFE (PFGE) System 8.0