Rotaphor 8 Pulsed Field Gel Electrophoresis (PFGE) System
- Excellent separation of large DNA up to 8 Mb
- Rotating electrodes for free selection of field angles
- Application flexibility: PFGE, CHEF, FIGE and ROFE
- Built-in circulation pump for buffer temperature control
- Easy to program, easy to run
- 17 preset programs for different size ranges
PFGE system for separation of large DNA molecules
Pulsed field gel electrophoresis with the Rotaphor 8 system allows the separation of DNA far beyond the 50 kb limit of conventional agarose electrophoresis. Thanks to the patented electrode rotor, the electrical field can be applied in virtually any angle. To achieve a very homogeneous field, the two main rotor electrodes are flanked by 2 sets of secondary electrodes each.
Buffer temperature control
PFGE gels typically run for many hours, even up to several days. Therefore, the electrophoresis buffer has to be cooled. The Rotaphor electrophoresis chamber has a built-in buffer circulation pump which is connected to an external cooling thermostat. During the run the buffer temperature in the electrophoresis chamber is constantly monitored and precisely controlled by the Rotaphor software.
The Rotaphor system includes a PC that controls the electrode rotor and the power supply over the Rotaphor interface card. The Rotaphor software provides 17 pre-set programs for separation of different size ranges. Starting with the pre-set parameters, new applications are quickly optimised. For each pre-set program the software shows a real gel image. Once a custom program has been optimised, a referring gel picture can be uploaded into the software. Thus, the Rotaphor software over time will not only be a control tool, but also a library of successful experiments linked to the underlying separation parameters. By linking programs to combined lists also complex sequences can be programmed.
The Rotaphor System includes:
- Electrophoresis chamber with electrode rotor and internal buffer circulation
- power supply
- gel tray
- comb (18 teeth)
- frame for gel casting
- sample mould
- computer with interface card and pre-installed Rotaphor software
- 22" TFT monitor
- connection cables
- gel scoop