GenUP Exo SAP PCR Cleanup Kit
- Simple protocol: 2 steps, 15 minutes, no columns
- 100% recovery
- Easily scaled and automated
- Economical alternative to other kits based on Exo SAP
ExoSAP Kit for easy and fast PCR Cleanup
The GenUP Exo SAP Kit from biotechrabbit allows quick and easy clean-up of PCR reactions for immediate use in downstream applications. It is fully enzymatic and therefore the method is scalable and ideal for automation.
The Exonuclease I (Exo) efficiently degrades single-stranded primers and Shrimp Alkaline Phosphatase (SAP) then dephosphorylates all nucleotides and primers so they will no longer interfere with sequencing, or genotyping.
Both enzymes are active in PCR buffers and completely inactivated by heating for 10 min at 80°C: it requires no further processing. The PCR product, consisting of double-stranded DNA, is not affected, ensuring 100% recovery.