innuPREP Plasmid DNA Mini Kit 2.0
- Isolate both high and low copy plasmids
- Use up to 15 ml of bacterial culture
- Fast protocol: 16 minutes
- Superior DNA yield: up to 70 µg
- Excellent DNA quality
innuPREP Plasmid Mini Kit 2.0 allows fast and easy isolation of plasmid DNA from bacteria cultures. It is an optimized version of the original innuPREP Plasmid kit and now allows for processing of 0.5 ml up to 15 ml of bacterial cultures. The new spin filter membrane yields up to 80 µg plasmid DNA from one sample.
The kit is based on the combination of an alkaline lysis and binding of the plasmid DNA to a filter membrane after precipitation of the chromosomal DNA and bacterial proteins. Bound plasmid DNA is then washed and finally eluted by using a low salt buffer. The isolated plasmid DNA can be used for a number of sensitive downstream applications, like transfection, cloning, PCR or sequencing.
Typical yields for high-copy plasmids:
- from 2 ml culture 6 - 20 µg
- from 15 ml culture: 60 - 70 µg