innuPREP Plant RNA Kit
- Ready-to-use plant RNA in 30 minutes after homogenization
- Selective removal of DNA from lysate
- No DNase digestion needed
- Two lysis buffers allow wide range of starting materials
- No toxic beta-mercaptoethanol
The innuPREP Plant RNA Kit is optimized for fast and efficient isolation of total RNA from up to 100 mg of plant ma terial.The underlying chemistry guarantees highly efficient lysis and effectively inactivates both endogenous and exogenous RNases.
Removal of contaminating genomic DNA does not require any DNase digestion. Instead, the genomic DNA is depleted from the lysate by a fast column capture step before the column purification of RNA.
Two lysis buffers are provided to allow you to process a wide range of fresh or frozen starting materials and species. For tough plant samples, like seeds or wood, it is recommended to use the SpeedMill bead homogenizer. The purification procedure is only 30-40 minutes and does not require any organic extractions or precipitations.
The binding capacity of the column is 100 µg. Actual yields can be up to 70 µg, but vary depending on the nature and amount of the sample.