innuPREP RNA MIDI Direct Kit
- An easy-to-use kit for isolating high-quality total RNA from a variety of different starting materials and quantities
- MIDI-format yields up to 500 µg RNA
- Genomic DNA removal step eliminates DNase I digestion
- Does not use toxic β-mercaptoethanol
The extraction procedure for the innuPREP RNA MIDI Direct Kit is based on a patented technology. After homogenizing and/or lysing the starting material, the next step is to remove the genomic DNA from the sample by efficiently binding it to an initial MIDI Spin Filter column. Adding ethanol to the RNA-containing filtrate binds the nucleic acid to a second MIDI Spin Filter where it is washed and finally eluted in RNase-free water. The optimized isolation chemistry inactivates both endogenous as well as exogenous RNases, thereby guaranteeing a final yield of intact, pure total RNA free of gDNA or other contaminants.