Phire & Phusion Direct PCR Kits

  • No DNA purification needed
  • Very fast PCR protocols
  • Including premixed gel loading dye
  • Mixes contain Phire Hot Start II DNA Polymerase
  • PCR's can be performed in the presence of animal tissue-derived inhibitors
  • For fresh or frozen samples

Direct PCR - no DNA purification needed

The Direct PCR Master mixes have been developed for direct DNA amplification from a wide variety of tissues obtained from animals, plants or humans. The mixes contain the Phire or Phusion Hot Start II DNA Polymerases that are specially formulated to perform PCR's in the presence of:

  • different tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle)
  • different plant material such as complex polysaccharides, polyphenols
  • inhibitors present in whole blood

Therefore, there is no need for DNA purification prior to your PCR setup.

Easy gel loading dye included

All Master Mixes include a premixed gel loading dye to minimize the possibility of cross-contamination, reduce your sample handling and allows direct loading on gel. The dye does not interfere with the PCR reaction.

For your convenience, you can also choose the Direct PCR Kits that include a complete set of optimized PCR reagents, detailed protocols for different sample types and universal control primers that are compatible with most species (human, plant or animal, depending on the kit).

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Thermo Scientific - Nucleic Acid Detection website to find your local distributor.
Cat no. Description
TH F-130WH Phire Plant Direct PCR Kit (without sampling tools) - 200 x 50µL rxns
TH F-140WH Phire Animal Direct PCR Kit (without sampling tools) - 200 x 50µL rxns
TH F-150 Phusion Human Specimen Direct PCR Kit - 200 x 20µL rxns
TH F-160L Phire Plant Direct PCR Master Mix - 500 x 50µl rxns
TH F-160S Phire Plant Direct PCR Master Mix - 100 x 50µl rxns
TH F-170L Phire Tissue Direct PCR Master Mix - 500 x 50µl rxns
TH F-170S Phire Tissue Direct PCR Master Mix - 100 x 50µl rxns
TH F-175L Phusion Blood Direct PCR Master Mix - 500 x 20µl rxns
TH F-175S Phusion Blood Direct PCR Master Mix - 100 x 20µl rxns
TH F-547L Phusion Blood Direct PCR Kit - 500 x 20µL rxns
TH F-547S Phusion Blood DirectPCR Kit - 100 x 20µL rxns