Terra Direct PCR Polymerase Mix
- Direct PCR on animal or plant tissues/extracts
- Save time and money on DNA isolation
- Efficient PCR in the presence of PCR inhibitors
- Amplify difficult GC-rich DNA effortlessly
- Unparalleled hot start antibody inhibits enzyme activity up to 98 C
Terra PCR Direct Polymerase Mix saves you time and money by allowing you to skip DNA isolation, and go directly to PCR amplification.
Terra PCR Direct contains a novel DNA polymerase that lets you amplify directly from animal and plant tissues, as well as from crude DNA extracts.
Moreover, Terra PCR Direct comes pre-blended with a monoclonal antibody for hot start PCR. Terra PCR Direct Polymerase Mix lets you easily amplify DNA targets up to 2 kb, even when the GC-content is greater than 70%.
Terra PCR Direct has been optimized for direct PCR amplification from animal and plant tissues, such as blood, tail and ear biopsies, or leaf cuttings.
Gel visualization trick:
PCR products amplified directly from animal tissues do not always resolve well on agarose gels. This can be easily prevented by adding proteinase K to your gel loading buffer. Proteinase K is now included with the Terra PCR Direct Polymerase Mix and the Terra PCR Direct Red Dye Premix to enhance gel detection. In the user manual you can see examples of how Proteinase K improves gel detection of Terra PCR Direct products.