High Fidelity PCR Enzyme Mix

  • 4x higher fidelity (vs Taq DNA Polymerase)
  • Long amplification products
  • High yields

High yield and fidelity of (RT)PCR amplification products

The High Fidelity PCR Enzyme Mix from Thermo Scientific is a unique blend of Taq DNA Polymerase and a thermostable DNA polymerase with proofreading activity. These two enzymes synergistically generate longer PCR amplification products with greater yield and more than four times higher fidelity than Taq DNA Polymerase alone.

Generation of long (RT)PCR products

Long amplicons are often difficult to generate with a single DNA polymerase. The High Fidelity PCR Enzyme Mix generates long amplicons of up to 20 kb with viral DNA templates and up to 10 kb with genomic DNA templates, both blunt-ended and 3'-dA tailed PCR products.

Applications

High Fidelity PCR Enzyme Mix can be used for:

  • Cloning purposes
  • Expression purposes
  • Site-directed mutagenesis
  • High yield PCR
  • Long range PCR
  • RT-PCR

Resources

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Thermo Scientific - Nucleic Acid Detection website to find your local distributor.
Cat no. Description
TH K0191 High Fidelity PCR Enzyme Mix - 100 Units
TH K0192 High Fidelity PCR Enzyme Mix - 500 Units