Phusion Polymerase

  • Phusion High-fidelity PCR Polymerase
  • Consistent high DNA yield
  • Fewer reaction failures
  • Minimal optimization
  • Fast polymerase, short reactions

Phusion Polymerase: robust and accurate high-fidelity PCR

Choose Phusion Polymerase from Thermo for highly accurate, fast and robust PCR. The enzyme, originally developed by Finnzymes, is a fusion of a proofreading polymerase and a processivity-enhancing domain, creating one of the most reliable and robust polymerases available.

Fast PCR with high fidelity

Compared to other polymerases Phusion DNA Polymerase incorporates more nucleotides before it falls off the DNA template. This "processivity" of Phusion DNA Polymerases is about 10 times greater than Pfu DNA polymerase and 2 times Taq DNA polymerase. This results in shorter extension times, stronger bands and the ability to amplify longer templates (up to 20 kb).

The shortest runs can be achieved with Phusion Flash High-Fidelity PCR Master Mix, a product developed specifically for fast PCR.

Go Green

PCR plate with Green master mix of Phusion PolymeraseThe new Phusion Green format is a combination of Phusion DNA Polymerase and 5x Green Reaction Buffer. This buffer includes a density reagent and two tracking dyes for direct loading of PCR products on gels when the reaction is finished.

The green buffer does not interfere with the performance of Phusion Polymerase during the reaction and is also compatible with applications after the PCR such as DNA sequencing, ligation or  digestion.


Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Thermo Scientific website to find your local distributor.
Cat no. Description
TH F-530L Phusion Polymerase - 500 U
TH F-530S Phusion Polymerase - 100 U
TH F-531L Phusion PCR Master Mix with HF Buffer - 500 x 50 µL rxns
TH F-531S Phusion PCR Master Mix with HF Buffer - 100 x 50 µL rxns
TH F-532L Phusion PCR Master Mix with GC Buffer - 500 x 50 µL rxns
TH F-532S Phusion PCR Master Mix with GC Buffer - 100 x 50 µL rxns
TH F-534L Phusion Green DNA Polymerase - 500 U
TH F-534S Phusion Green DNA Polymerase - 100 U
TH F-548L Phusion Flash High-Fidelity PCR Master Mix - 500 x 20 rxns
TH F-548S Phusion Flash High-Fidelity PCR Master Mix - 100 x 20 rxns
TH F-553L Phusion High-Fidelity PCR Kit - 200 x 50 µL rxns
TH F-553S Phusion High-Fidelity PCR Kit - 50 x 50 µL rxns