- High-fidelity PCR Polymerase
- Consistent high yields
- Fewer reaction failures
- Minimal optimization
- Fast polymerase, short reactions
Phusion Polymerase: robust and accurate high-fidelity PCR
Choose Phusion Polymerase from Thermo for highly accurate, fast and robust PCR. The enzyme, originally developed by Finnzymes, is a fusion of a proofreading polymerase and a processivity-enhancing domain, creating one of the most reliable and robust polymerases available.
Fast PCR with high fidelity
Compared to other polymerases Phusion DNA Polymerase incorporates more nucleotides before it falls off the DNA template. This "processivity" of Phusion DNA Polymerases is about 10 times greater than Pfu DNA polymerase and 2 times Taq DNA polymerase. This results in shorter extension times, stronger bands and the ability to amplify longer templates (up to 20 kb).
The shortest runs can be achieved with Phusion Flash High-Fidelity PCR Master Mix, a product developed specifically for fast PCR.
The new Phusion Green format is a combination of Phusion DNA Polymerase and 5x Green Reaction Buffer. This buffer includes a density reagent and two tracking dyes for direct loading of PCR products on gels when the reaction is finished.
The green buffer does not interfere with the performance of Phusion Polymerase during the reaction and is also compatible with applications after the PCR such as DNA sequencing, ligation or digestion.