Phusion U Hot Start DNA Polymerase

  • 25x higher fidelity vs Taq DNA polymerase
  • Incorporates dUTP and amplifies uracil containing templates
  • Short extension times (15-30 s/kb)
  • Amplification of long amplicons (<20 kb)
  • High specificity with Affibody based hot start

Activated Phusion U Hot Start DNA polymerase

The Phusion U Hot Start DNA Polymerase is an enzyme that is combined with a reversibly bound, specific Affibody protein. Binding of the Affibody protein inhibits DNA polymerase and 3’-5’ exonuclease activities at ambient temperatures, which allows you easy pipetting. Moreover, usage of Phusion U Hot Start DNA Polymerase avoids nonspecific amplification and primer degradation.

When polymerase activity temperature is reached, the Affibody ligand is released, hereby activating the enzyme. Due to a proprietary mutation, Phusion U Hot Start DNA Polymerase is able to incorporate dUTP and read through uracil present in DNA templates. In combination with its great accuracy, speed, ability to amplify long amplicons and a high specificity with the Affibody based hot start, the enzyme is an excellent choice for amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.

Broad application possibilities

You can use the Phusion U Hot Start DNA Polymerase for a wide variety of applications::

  • Amplification of bisulphite-converted DNA
  • Amplification of damaged or aged DNA
  • Carry-over contamination control
  • Uracil-excision based (USER) cloning methods

Ready-to-use PCR master mixes and green buffer

Phusion U Hot Start DNA Polymerase is also available in the Master Mix and Green formats. The 2X Phusion U Hot Start PCR Master Mix minimizes the number of pipetting steps.

The Phusion U Green formats are a combination of the enzyme and 5X Phusion Green Buffers that include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The green color does not interfere with the performance of the enzyme.
 

Resources

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Thermo Scientific - Nucleic Acid Detection website to find your local distributor.
Cat no. Description
TH F-533L Phusion U Hot Start PCR Master Mix - 500 x 50 µL
TH F-533S Phusion U Hot Start PCR Master Mix - 100 x 50 µL
TH F-555L Phusion U Hot Start DNA Polymerase - 500 Units
TH F-555S Phusion U Hot Start DNA Polymerase - 100 Units
TH F-556L Phusion U Green Hot Start DNA Polymerase - 500 Units
TH F-556S Phusion U Green Hot Start DNA Polymerase - 100 Units