Pyrobest DNA Polymerase

  • High fidelity: comparable to Pfu
  • Robust amplification

Pyrobest is an α-type thermostable DNA polymerase derived from Pyrococcus sp., which possesses an associated 3'-5’exonuclease (proofreading) activity. Pyrobest is a highly suitable enzyme for high-fidelity amplification thanks to the high proofreading activity.

Generally it is considerd that α-type DNA polymerase is more difficult to use for PCR than PolI-type DNA polymerase (such as Taq polymerase), because the optimum reaction conditions of α-type DNA polymerase is relatively narrow.
However, Pyrobest is able to offer productivity as good as Taq polymerase, as a result of buffer optimization. At least 5 kb can be amplified from human genomic DNA template or λ-DNA template using Pyrobest.

Resources

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Takara website to find your local distributor.
Cat no. Description
TB R005A Pyrobest DNA Polymerase, 125u