Maxima Hot Start Taq DNA Polymerase

  • Inactive at RT
  • Short 4 min activation step (Hot Start)
  • High specificity and sensitivity
  • Amplification of <3 kb genomic DNA
  • Broad application possibilities

Room Temperature Hot Start PCR set-up

The Maxima Hot Start Taq DNA Polymerase is chemically modified in such a way that the enzyme is inactive at room temperature. Hereby, you avoid the extension of non-specifically annealed primers or primer dimers and you can easily pipet your PCR reaction at room temperature. You activate the enzyme by a short 4 minute incubation step at 95°C, prior to your PCR reaction. The activated enzyme maintains the same functionality as Taq DNA polymerase.

Broad application possibilities

You can use the Maxima Hot Start Taq DNA Polymerase for a wide variety of applications:

  • Hot start PCR
  • RT-PCR
  • Highly specific amplification of complex genomic and cDNA templates up to 3 kb
  • Amplification of low copy DNA targets
  • Real-time PCR
  • Multiplex PCR
  • Generation of PCR products for TA cloning

The enzyme provides higher specificity of DNA amplification.

Ready-to-use PCR master mixes

For easy use, ready-to-use PCR Master MIxes including the Maxima Hot Start Taq DNA Polymerase are also available. Beside the enzyme, the Master mixes include hot start PCR buffer, dNTPs and Magnesium.These solutions will save you pipetting time.

For even more convenience, you can use the Maxima Hot Start Green PCR Master Mix. This mix also includes two tracking dyes and a density reagent that allows for direct loading of PCR products on a gel. The dyes in the master mix do not interfere with PCR performance.

 

Specific amplification with Maxima Hot Start Taq DNA Polymerase

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Thermo Scientific - Nucleic Acid Detection website to find your local distributor.
Cat no. Description
TH EP0601 Maxima Hot Start Taq DNA Polymerase - 100 U
TH EP0602 Maxima Hot Start Taq DNA Polymerase - 500 U
TH EP0603 Maxima Hot Start Taq DNA Polymerase - 5 x 500 U
TH K1051 Maxima Hot Start PCR Master Mix (2X) - 100 x 50µL rxns
TH K1052 Maxima Hot Start PCR Master Mix (2X) - 500 x 50µL rxns
TH K1061 Maxima Hot Start Green PCR Master Mix (2X) - 100 x 50 ?L rxns
TH K1062 Maxima Hot Start Green PCR Master Mix (2X) - 500 x 50 ?L rxns