Phire Hot Start II DNA Polymerase

  • Quick hot start – no reactivation step
  • Fast – 4x faster than hot start Taq
  • Minimal PCR optimization
  • High yield of PCR products
  • Allows PCR of longer products
  • Green version: direct gel loading

Very, very fast PCR

Thermo Scientific Phire Hot Start II DNA Polymerase incorporates a dsDNA-binding domain that allows short extension times (10-15 s/kb), improves yields, and increases fidelity two-fold compared to Taq DNA polymerase. In addition, the unique hot start technology allows complete reactivation of the enzyme in “zero-time” at
standard cycling temperatures.

This combination of features makes Phire Hot Start II DNA Polymerase an ideal solution for routine and high-throughput PCR applications. 

It's easy, being green

Phire Green Hot Start II DNA Polymerase is supplied with 5x Phire Green Reaction Buffer that includes a density reagent and two tracking dyes for direct loading of PCR products on gels.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Thermo Scientific - Nucleic Acid Detection website to find your local distributor.
Cat no. Description
TH F-122L Phire Hot Start II DNA Polymerase - 1000 x 50 µL rxns
TH F-122S Phire Hot Start II DNA Polymerase - 200 x 50 µL rxns
TH F-124L Phire Green Hot Start II DNA Polymerase - 1000 x 50 µl rxns
TH F-124S Phire Green Hot Start II DNA Polymerase - 200 x 50 µl rxns
TH F-125L Phire HSII PCR Master Mix 1000 x 50 µl rxns
TH F-125S Phire HSII PCR Master Mix 200 x 50 µL rxns
TH F-126L Phire Green HSII PCR Master Mix 1000 x 50 µl rxns
TH F-126S Phire Green HSII PCR Master Mix 200 x 50 µl rxns