Phoenix Hot Start Taq DNA Polymerase
Phoenix Hot Start Taq DNA Polymerase is a recombinant, thermostabile Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the 5’ 3’ polymerase activity prior to the initial DNA denaturation step of PCR.
Such antibody-mediated Hot-Start capability enhances the overall specificity, sensitivity and yield of the PCR by reducing nonspecific amplification and primer-dimer formation prior to PCR cycling, and allows the convenience of reaction set up at room temperature. When the temperature of the PCR reaction mix reaches ≥94°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored.
Phoenix Hot Start Taq DNA Polymerase, like standard Taq DNA polymerase, also has 5’-3’ exonuclease activity, but lacks any detectable 3’-5’ exonuclease activity.