Phoenix Hot Start Taq DNA Polymerase

Phoenix Hot Start Taq DNA Polymerase is a recombinant, thermostabile Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the 5’ 3’ polymerase activity prior to the initial DNA denaturation step of PCR.

Such antibody-mediated Hot-Start capability enhances the overall specificity, sensitivity and yield of the PCR by reducing nonspecific amplification and primer-dimer formation prior to PCR cycling, and allows the convenience of reaction set up at room temperature. When the temperature of the PCR reaction mix reaches ≥94°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored.

Phoenix Hot Start Taq DNA Polymerase, like standard Taq DNA polymerase, also has 5’-3’ exonuclease activity, but lacks any detectable 3’-5’ exonuclease activity. 

Resources

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the MB Enzymes website to find your local distributor.
Cat no. Description
EZ P7590L Phoenix Hot Start Taq DNA Polymerase 500 units (5,000 U/ml)
EZ P7590S Phoenix Hot Start Taq DNA Polymerase 100 units (5,000 U/ml)