TruePrime WGA Kit

  • Whole genome amplification without synthetic primers
  • No primer artefacts or bias
  • Excellent coverage
  • High sensitivity as low as 1 fg DNA
  • High reproducibility

Primer-free amplification of low input purified DNA

TruePrime™ WGA kit uses a revolutionary novel multiple displacement amplification method based on the combination of the recently discovered DNA primase TthPrimPol and the extremely processive and high-fidelity Phi29 DNA polymerase to amplify uniformly total genomic DNA from purified material. The extraordinary strand displacement capacity of the Phi29 DNA polymerase allows TthPrimPol to generate new primers on the displaced strands that are extended by Phi29 DNA pol, resulting in exponential isothermal DNA amplification.

Excellent genome coverage

The yeast genome was amplified with TruePrime™ technology and sequenced on an Illumina sequencer. Using 2.5 million read pairs 99.4% of the total yeast genome was covered. The graph shows the evenness of coverage with a narrow distribution curve.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Expedeon website to find your local distributor.
Cat no. Description
EP 370025 TruePrime WGA Kit (25 reactions)
EP 380100 TruePrime WGA Kit (100 reactions)