TruePrime Single-Cell WGA Kit
- Single-cell whole genome amplification
- No primer artefacts or bias
- Excellent coverage, similar to non-amplified DNA
- Extreme sensitivity down to femtograms DNA
- High reproducibility gives maximum data confidence
TruePrime technology: primer-free DNA amplification
The TruePrime Single-Cell WGA kit incorporates SYGNIS’ revolutionary TruePrime™ multiple displacement amplification (MDA) technology. This combines Phi29 DNA polymerase-based whole genome amplification with TthPrimPol, a novel enzyme that can synthesize primers randomly on single-stranded target DNA.
No primer artefacts
The absence of random synthetic primers prevents any amplification artefacts that might be generated by random extension of primer dimers, etc. When 1 pg of human genomic DNA (~ 1/6th of the DNA content of one mammalian cell was amplified using either TruePrime™ or random primed MDA reactions the random primed reactions contain 20% of sequences that cannot be mapped to any organism in sequence databases. With TruePrime >99% of sequences was target derived, and even with 1 fg DNA input this figure is >95%.
Superior coverage of the genome, very low bias
TruePrime-amplified DNA shows xcellent overall mapping data, closest to non-amplified DNA coverage. Single Hek293 cells were amplified with different MDA-type single cell amplification methods and coverage was compared to the theoretically possible coverage (Poisson) and the coverage in non-amplified DNA from the same Hek293 population. Exactly 12 million read pairs were mapped to the human genome. Below the coverage graph for chromosome 3 depicting differences in evenness of coverage between the methods. Note the extreme similarity between non-amplified and TruePrime.