LA Taq DNA Polymerase

  • Long and Accurate Amplification due to LA PCR technology.
  • 20 kbp extension with ease and up to 48 kbp amplification with PCR kit version 2.1.
  • Suitable for Genomic DNA Amplification and applicable for GC Rich and comlex templates.
  • Hot start version ena
TaKaRa LA Taq polymerase is a thermostable DNA polymerase with 3′ → 5′ exonuclease proof reading activity utilizing Long and Accurate PCR technology. Higher efficiency and higher fidelity are obtained compared than conventional Taq DNA polymerases. This enzyme is basically applicable to any template DNA, and especially effective in amplifying more than 15 kb fragments. For amplification of templates having complex secondary structure or high GC content, TaKaRa LA Taq polymerase with GC Buffer works the best. The supplied 2 × GC Buffer I and II are designed to achieve the best amplification of GC-rich templates or templates with high repeat sequences.

In addition TaKaRa LA Taq Hot Start polymerase is designed for Hot Start PCR. It is derived from TaKaRa LA Taq polymerase with the addition of a neutralizing monoclonal antibody. Non-specific amplification due to mispriming and/or formation of primer dimer before thermal cycling can be prevented. The hot start antibody inhibits the polymerase activity by binding to the Taq DNA polymerase until the temperature elevates. No adjustment of thermal cycler conditions is required.

For Long and Accurate PCR Amplification of targets up to 48 kbp, LA PCR Kit Ver.2.1. is designed. Using TaKaRa LA Taq polymerase in conjunction with an optimized reaction buffer enables to generate longer and more accurate PCR products with greater yields. LA PCR Kit Ver.2.1 includes all the reagents required for optimal Long and Accurate PCR in terms of efficient amplification, high fidelity and long PCR product yield. LA PCR technology is expected to be of particular value in facilitating genome analysis in various ways. Moreover, by using supplied specially designed GC buffer this kit is applicable to amplify GC rich templates.

The One Shot LA PCR Mix Ver. 2.0 is an optimized mixture composed of TaKaRa LA Taq polymerase, reaction buffer and dNTP mixture as 2-fold concentration. For the convenience the One Shot LA PCR Mix ver. 2.0 is dispensed in 0.2 ml PCR tubes to allow the user to start PCR only by adding template and primers.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Takara website to find your local distributor.
Cat no. Description
TB RR002A TaKaRa LA Taq (Mg free buffer), 125u
TB RR002M TaKaRa LA Taq (Mg plus buffer), 250u
TB RR004 One Shot LA PCR Mix, 24rxns
TB RR013A TaKaRa LA PCR Kit Ver.2.1, 50rxns
TB RR02AG TaKaRa LA Taq with GC Buffer, 125u
TB RR042A TaKaRa LA Taq Hot Start, 125u