50 mM MgCl2
- PCR-grade MgCl2 solution
- Optimization of PCR buffer conditions
Many applications use the standard concentration of 1.5 mM MgCl2. However, reactions with higher amounts of template (such as genomic DNA), primers and nucleotides might require higher MgCl2 concentrations (2–3 mM). The 50 mM MgCl2 solution can be used to adjust magnesium chloride to the optimal concentration.