PCR Carryover Prevention Kit

The TaKaRa PCR Carryover Prevention Kit is designed to preclude false-positive results caused by carryover contamination. This product includes dUTP instead of dTTP for PCR. It also includes UNG (uracil-N-glycosylase), an enzyme that degrades uracil-containing DNA.

Treatment with UNG prior to PCR degrades uracil-containing PCR contaminants but leaves thymine-containing templates intact,
allowing selective removal of carryover PCR products.

Degradation by UNG takes place in the following manner. In a pre-PCR reaction at 25℃ for 10 min,
UNG hydrolyzes N-glycosylic bonds between the deoxyribose sugars and the uracil bases in uracil-containing DNA, leaving apyrimidinic sites in the DNA.

Next, a heat treatment at 95℃ for 2 min deactivates UNG and simultaneously causes cleavage and degradation of the contaminant DNA
fragments by hydrolysis of the phosphate backbone at the abasic sites.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Takara website to find your local distributor.
Cat no. Description
TB 6088 PCR Carryover Prevention Kit, 200rxns