SpeedTaq PCR Optimization Set

New PCR assays generally have to be optimized to get the highest PCR yield specificity. The SpeedTaq Optimization Set is a fast ans easy tool to optimize PCR conditions.

Each Optimization Set contains a PCR enzyme optimized for rapidPCR (standard or hot start), and a set of 10 different buffers delivered in pH 8 and pH 9. The complexity of PCR optimization can is reduced to a minimum without any trouble and loss of quality. Just a few steps have to be performed to get the expected results.



Step 1: Prepare PCR master mix with target DNA and primers
Step 2: combine with each of the 10 buffers and run the amplification
Step 3: analyze on gel to find the best performing buffer
Step 4: for subsequent assays use this buffer. Each PCR buffer is available separately.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Analytik Jena website to find your local distributor.
Cat no. Description
AJ 845-AK-1100100 10x SpeedAmp Optimization Buffer Kit - 0.10 ml
AJ 845-AK-1100250 10x SpeedAmp Optimization Buffer Kit - 0.25 ml
AJ 845-AK-1100500 10x SpeedAmp Optimization Buffer Kit - 0.5 ml
AJ 845-AK-1200500 10x SpeedAmp Optimization Buffer 1, pH8 - 0.5 ml
AJ 845-AK-1200501 10x SpeedAmp Optimization Buffer 1, pH9 - 0.5 ml
AJ 845-AK-1201000 10x SpeedAmp Optimization Buffer 1, pH8 - 1.0 ml
AJ 845-AK-1201001 10x SpeedAmp Optimization Buffer 1, pH9 - 1.0 ml
AJ 845-AK-1300500 10x SpeedAmp Optimization Buffer 2, pH8 - 0.5 ml
AJ 845-AK-1300501 10x SpeedAmp Optimization Buffer 2, pH9 - 0.5 ml
AJ 845-AK-1301000 10x SpeedAmp Optimization Buffer 2, pH8 - 1.0 ml
AJ 845-AK-1301001 10x SpeedAmp Optimization Buffer 2, pH9 - 1.0 ml
AJ 845-AK-1400500 10x SpeedAmp Optimization Buffer 3, pH8 - 0.5 ml
AJ 845-AK-1400501 10x SpeedAmp Optimization Buffer 3, pH9 - 0.5 ml
AJ 845-AK-1401000 10x SpeedAmp Optimization Buffer 3, pH8 - 1.0 ml
AJ 845-AK-1401001 10x SpeedAmp Optimization Buffer 3, pH9 - 1.0 ml
AJ 845-AK-1500500 10x SpeedAmp Optimization Buffer 4, pH8 - 0.5 ml
AJ 845-AK-1500501 10x SpeedAmp Optimization Buffer 4, pH9 - 0.5 ml
AJ 845-AK-1501000 10x SpeedAmp Optimization Buffer 4, pH8 - 1.0 ml
AJ 845-AK-1501001 10x SpeedAmp Optimization Buffer 4, pH9 - 1.0 ml
AJ 845-AK-1600500 10x SpeedAmp Optimization Buffer 5, pH8 - 0.5 ml
AJ 845-AK-1600501 10x SpeedAmp Optimization Buffer 5, pH9 - 0.5 ml
AJ 845-AK-1601000 10x SpeedAmp Optimization Buffer 5, pH8 - 1.0 ml
AJ 845-AK-1601001 10x SpeedAmp Optimization Buffer 5, pH9 - 1.0 ml
AJ 845-EZ-1110100 SpeedTaq Optimization Set - 50 Units, 10 Buffers 100µl each
AJ 845-EZ-1120100 SpeedTaq HOT Optimization Set - 50 Units, 10 Buffers 100µl each