CellAmp Whole Transcriptome Amplification Kit
- Directly amplify of cDNA from cells.
- amplification of cDNA from small amount of RNA.
The CellAmp Whole Transcriptome Amplification kit is designed to perform cDNA amplification directly from small amounts of cell. The amplified cDNA can be applied for real-time PCR as a template. In general, when dealing with small amounts of nucleic acid there is a problem in loss of nucleic acids during the purification process. However, by using the CellAmp kit cDNA can be amplified efficiently without purifying RNA or cDNA. The first step is cell lysis, followed by cDNA synthesis from mRNA by reverse transcription using dT adapter primer. Next, dA tails are added by TdT, The synthesized cDNA will be used as a template for low cycle PCR to amplify the cDNA.