PrimeScript II 1st strand cDNA Synthesis Kit
- Increase specific binding of reverse transcriptase due to improved enzyme
- Easily runs through RNA with high secondary structure
- Efficient RT at 42°C prevents RNA damage at higher temperatures
- More efficient cDNA synthesis means higher sensitivity
PrimeScript II 1st strand cDNA Synthesis Kit contains all the reagents necessary to synthesize 1st strand cDNA from total or poly(A)+ RNA using PrimeScript II RTase. In the case of complex RNA structure or long-length RNA as a template, non-specific binding of reverse transcriptase to higher structure RNA interferes with appropriate cDNA synthesis. In addition, non-specific elongation due to mis-priming of the reverse transcriptase can cause problems in RT-PCR or production of full-length cDNA. PrimeScript II RTase, which contains accessory protein similar to PrimeScript RTase developed by TaKaRa Bio, is a reverse transcriptase which decreases these problems in cDNA synthesis reaction. This enzyme synthesizes full-length cDNA products efficiently at standard temperatures (42°C). 1st strand cDNAs synthesized with this kit can be used for variety of applications including 2nd strand synthesis, hybridization, and PCR amplification. Furthermore, this kit is useful for application to construct high-quality, full-length cDNA.