PrimeScript One Step RT-PCR Kit version 2

  • Including all components necessary for RT-PCR in a sigle tube, minimizing the risk of contamination
  • Robust and Efficient assay due to the combination of PrimeScript Reverse transcriptase and TaKaRa Ex Taq HS polymerase.
  • poduces full-length cDNAs up to
PrimeScript One Step RT-PCR Kit Version 2 is designed to perform RT-PCR and PCR in a single tube. featuring Primescript reverse transcriptase a novel reverse transcriptase, developed by TaKaRa Bio based on MMLV reverse transcriptase. This enzyme offers robust reverse transcription on any RNA template and excellent extendibility for RNA templates containing higher-order structures.

PrimeScript RTase works well at 50 °C temperature on challenging templates, therefore high temperature transcription, which increases the risk of RNA degradation is not necessary.

Furthermore The PrimeScript One Step RT-PCR kit version 2 also includes the TaKaRa hot start PCR enzyme, TaKaRa Ex Taq HS, for efficient, sensitive and specific amplification of the cDNA.

Combining the TaKaRa Bio PrimeScript RTase with the Ex Taq HS polymerase, reliable and reproducible RT-PCR amplification from a wide variety of RNA templates is achieved. The One Step enzyme mix provides simple and efficient reaction assembly. PrimeScript One Step RT-PCR kit Version 2 achieves a simple and convenient experimental procedure by using all components required in a premix format minimizing the risk of contamination.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Takara website to find your local distributor.
Cat no. Description
TB RR055A PrimeScript One Step RT-PCR Kit ver.2, 50rxns
TB RR057A PrimeScript One Step RT-PCR Kit ver. 2 (dye Plus), 50rxns