PrimeScript RT-PCR Kit
- Powerful two step RT-PCR
- Efficient complete elongation of high order RNA templates and high yield of full-length cDNA of up to 12 kb
- Complete elongation of complex RNA templates possible at 42 °C reverse transcription temperature
- Redused mispriming and
The PrimeScript RT-PCR Kit is a kit for two step RT-PCR designed to provide excellent extendibility and efficient amplification. The PrimeScript reverse transcriptase is developed by TaKaRa Bio Inc. based on RTase originated from MMLV, which provides complete elongation any RNA template even containing high order structures. This enzyme works well on challenging templates at 42 °C, making high temperature reverse transcription, which results in RNA degradation unnecessary. The PrimeScript RT-PCR kit also includes the TaKaRa Ex Taq HS polymerase, a hot start enzyme, which enables amplification with high efficiency, sensitivity and specificity. This combination of PrimeScript RTase and Ex Taq polymerase results in a more efficient gain, reliable and reproducible RT-PCR reaction from a large variety of RNA templates. This kit includes all reagents necessary for the reverse transcription of RNA to cDNA and cDNA amplification for end-point PCR.