DreamTaq Green DNA Polymerase

  • Optimized for high throughput PCR
  • Included dye allows direct loading on gel
  • High yields and high sensitivity
  • Robust amplification with minimal optimization
  • For routine PCR amplification of <6 kb DNA fragments

Fast loading due to included dyes

DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes, so you can directly load your PCR product on your gel. This allows you to perform high throughput PCR. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

The buffer includes a blue and a yellow dye. The blue dye migrates with 3-5 kb DNA fragments in a 1% agarose gel and the yellow dye migrates faster than 10 bp DNA fragments in 1% agarose gel.

DreamTaq Green DNA Polymerase features

With the DreamTaq Green DNA Polymerase you can amplify genomic DNA fragments of up to 6kb and viral DNA fragments of up to 20 kb. The polymerase incorporates modified nucleotides, but does not incorporate dUTP. As this product ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA Polymerases, extensive optimization of reaction conditions is not required.

Amplify longer PCR fragments and get higher yields with DreamTaq Green Polymerase

Easy complete PCR mix saves you time

The DreamTaq Green PCR Master Mix is a ready-to-use solution containing everything you need to perform your PCR reactions: DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs. This saves you time on pipetting, whereas all features of the DreamTaq Green DNA Polymerase are retained.

Resources

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Thermo Scientific - Nucleic Acid Detection website to find your local distributor.
Cat no. Description
TH B71 10X DreamTaq Green Buffer - 4 x 1.25 mL
TH EP0711 DreamTaq Green DNA Polymerase - 200 U
TH EP0712 DreamTaq Green DNA Polymerase - 500 U
TH EP0713 DreamTaq Green DNA Polymerase - 5 x 500 U
TH EP0714 DreamTaq Green DNA Polymerase - 20 x 500 U
TH K1081 DreamTaq Green PCR Master Mix (2X) - 200 x 50 µL rxns
TH K1082 DreamTaq Green PCR Master Mix (2X) - 1000 x 50 µL rxns