PrimeScript RT Reagent Kit (Perfect Real-Time)
- High-yield first strand cDNA synthesis for qRT-PCR
- Easily runs through RNA with high secondary structure at 42°C
- More efficient cDNA synthesis means higher sensitivity
- Reaction completed in 15 minutes
High cDNA yield at 42°C even for GC-rich targets
PrimeScript is unique MMLV-derived reverse transcriptase engineered to have a higher strand displacement activity. This activity easily "melts" regions of high secondary structure allowing the RT reaction to proceed. This means you can do the RT reaction at 42°C, and avoid the RNA damage occurs at 50-55°C, temperatures required for other enzymes. With PrimeScript RT GC-rich sequences are no longer a problem.
Fast synthesis of full-size cDNAs up to 12 kb
PrimeScript RT also stands out with its high extension rate. It also lacks RNase H activity preventing premature RNA degradation. With these properties the enzyme is able to synthesize full length cDNA of up to 12 kb in length with good yield.
Furthermore, the RT reaction is completed in just 15 minutes, which speeds up your qRT-PCR workflow.
The PrimeScript RT Reagent Kit contains everything you need for a perfect RT reaction:
PrimeScript Reverse Transcriptase
Oligo-dT and random 6-mer primers
Dilution buffer for subsequent qPCR