Luminaris Color HiGreen qPCR Master Mixes
- Ready-to-use solutions
- Easy pipetting dyes included
- Hot Start Taq DNA polymerase included
- No sequence-specific probes needed
- Elimination of non-specific amplification and primer dimer formation
Master Mixes including easy pipetting dyes
The Luminaris Color HiGreen qPCR Master Mixes hold the exact same features as the Luminaris HiGreen qPCR Master Mixes, but are supplemented with an inert blue Master Mix dye and a separate Yellow Sample Buffer that contains a yellow dye. Inclusion of the dyes allows for easy pipetting.
Ready-to-use solutions for qPCR and 2-step RT-qPCR
The Master Mixes are ready-to-use solutions that are optimized for qPCR and 2-step RT-qPCR. The master mixes include a Hot Start Taq DNA Polymerase in an optimized PCR buffer to ensures you PCR specificity and sensitivity. In addition, uracil-DNA glycosylase (UDG) and dNTPs are also included in the buffer for carry-over contamination control.
The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes. To set up your PCR reaction, you only need to add your template and primers.
Wide users range
The Master Mixes can be used in real-time PCR for the specific quantification of genomic, plasmid, viral, and cDNA templates. In combination with the optimized buffer, this product ensures PCR specificity and sensitivity as it detects single copy number targets and eliminates non-specific amplification and formation of primer dimers.
The Luminaris Color HiGreen qPCR Master Mixes are available with and without different concentrations of premixed ROX reference dye or with a fluorescein reference dye. Both dyes are suitable for most real-time PCR platforms.