Luminaris Color HRM Master Mix
- Ready-to-use master mix for HRM analysis method
- Dual-color system for easy reaction setup
- Superior discrimination of all four SNP classes
- Highly sensitive
- For high-throughput applications
Ready-to-use Master Mix for HRM analysis
With the Luminaris Color HRM Master Mix you can accurately detect DNA sequence variations using High Resolution Melt (HRM) analysis method. The master mix is a convenient, ready-to-use mix including all components needed for your complete HRM analysis.
Room temperature set-up with optimized buffer
This complete mix includes a chemically modified Hot Start Taq DNA polymerase that is inactive at room temperature, hereby avoiding the extension of non-specifically annealed primers or primer-dimers and providing higher specificity of DNA amplification. It provides you the convenience of reaction setup at room temperature. Due to its ultra low residual activity until thermally activated, which ensures you PCR specificity and sensitivity.
The enzyme is included in an optimized buffer system together with EvaGreen fluorescent dye for robust and reliable performance. The EvaGreen dye enables discrimination of even the most challenging sequence differences (such as Type IV SNP) without PCR inhibition and with no apparent sequence preference.
Easy pipetting dyes included
In addition to earlier described components, the Luminaris Color HRM Master Mix is supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. Mixing both components in a reaction turns the solution green. This provides a visual aid when pipetting and decreases the risk of errors during reaction setup.
You can use the Luminaris Color HRM Master Mix for a variety of applications:
- SNP genotyping
- Mutation discovery (gene scanning)
- Heterozygosity screening
- Bacterial genotyping
- CpG methylation analysis
The Master Mix shows high performance on most real-time instruments with HRM capabilities and complements Thermo Scientific PikoReal Real-Time System and software to provide a complete solution for HRM analysis.