Maxima Probe qPCR Master Mixes
- Ready-to-use solutions
- High reproducibility
- High specificity
- Elimination of non-specific amplification
- Optimized for use with sequence-specific probes
The Maxima Probe qPCR Master Mixes are ready-to-use solutions that are optimized for quantitative real-time PCR and two-step real-time RT-PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer and supplemented with a reference dye to serve as an internal reference for normalization. You only need to add template and primers. The buffer provides high specificity of primer annealing.
Wide users range
You can use the Probe qPCR Master Mixes in real-time PCR for the specific quantification of genomic, plasmid, viral, and cDNA templates. In combination with the optimized buffer, this product ensures PCR specificity and sensitivity as it detects low copy number targets and eliminates non-specific amplification and formation of primer dimers. In addition, dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG).
Internal control dye included
The mix includes the ROX reference dye that has a different emmission spectrum compared to the fluorescent dyes used for probes, so it does not interfere with your qPCR results. Rox allows for correction of well-to-well variation due to pipetting inaccuracies and fluorescence fluctuations. There is also a Probe qPCR Master mix available with the ROX solution seperately provided.
Probe qPCR Master mixes are compatible with most real-time thermal cyclers.