Luminaris HiGreen qPCR Master Mix

  • Ready-to-use solutions
  • Optimized for qPCR and 2-step RT-qPCR
  • Hot Start Taq DNA polymerase included
  • No sequence-specific probes needed
  • Elimination of non-specific amplification
  • Elimination of primer dimer formation

Ready-to-use solutions

The Luminaris HiGreen qPCR Master Mixes are ready-to-use solutions that are optimized for qPCR and 2-step RT-qPCR. The master mixes include a Hot Start Taq DNA Polymerase in an optimized PCR buffer to ensures you PCR specificity and sensitivity. In addition, uracil-DNA glycosylase (UDG) and dNTPs are also included in the buffer for carry-over contamination control. 

The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes. To set up your PCR reaction, you only need to add your template and primers.

Wide users range

The Luminaris HiGreen qPCR Master Mixes can be used in real-time PCR for the specific quantification of genomic, plasmid, viral, and cDNA templates. In combination with the optimized buffer, this product ensures PCR specificity and sensitivity as it detects single copy number targets and eliminates non-specific amplification and formation of primer dimers.

The Luminaris HiGreen qPCR Master Mixes are available with and without different concentrations of premixed ROX reference dye or with a fluorescein reference dye. Both dyes are suitable for most real-time PCR platforms.

Application possibilities

The Luminaris Probe qPCR Master Mixes can be used for a variety of applications:

  • Gene expression analysis
  • siRNA validation
  • Genotyping
  • Pathogen detection

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Thermo Scientific - Nucleic Acid Detection website to find your local distributor.
Cat no. Description
TH K0961 Luminaris HiGreen High ROX qPCR Master Mix - 250 x 20µL rxns
TH K0962 Luminaris HiGreen High ROX qPCR Master Mix - 500 x 20µL rxns
TH K0963 Luminaris HiGreen High ROX qPCR Master Mix - 1,250 x 20µL rxns
TH K0964 Luminaris HiGreen High ROX qPCR Master Mix - 5,000 x 20µL rxns
TH K0971 Luminaris HiGreen Low ROX qPCR Master Mix - 250 x 20µL rxns
TH K0972 Luminaris HiGreen Low ROX qPCR Master Mix - 500 x 20µL rxns
TH K0973 Luminaris HiGreen Low ROX qPCR Master Mix - 1,250 x 20µL rxns"
TH K0974 Luminaris HiGreen Low ROX qPCR Master Mix - 5,000 x 20µL rxns"
TH K0981 Luminaris HiGreen Fluorescein qPCR Master Mix - 250 x 20µL rxns
TH K0982 Luminaris HiGreen Fluorescein qPCR Master Mix - 500 x 20µL rxns
TH K0983 Luminaris HiGreen Fluorescein qPCR Master Mix - 1,250 x 20µL rxns
TH K0984 Luminaris HiGreen Fluorescein qPCR Master Mix - 5,000 x 20µL rxns
TH K0991 Luminaris HiGreen qPCR Master Mix - 250 x 20µL rxns
TH K0992 Luminaris HiGreen qPCR Master Mix - 500 x 20µL rxns
TH K0993 Luminaris HiGreen qPCR Master Mix - 1,250 x 20µL rxns
TH K0994 Luminaris HiGreen qPCR Master Mix - 5,000 x 20µL rxns