QPCR Reference RNA & cDNA

  • Standardize expression data from different experiments
  • High gene representation
  • Consistent results
  • Made from human tissues, not cultured cell lines
Measurements of mRNA expression levels are usually standardized by comparing the data to an internal reference gene. Housekeeping genes such as beta-actin or GAPDH are most often used because their expression levels are expected to remain constant under different treatment conditions. Unfortunately, this assumption is not always valid, and results based on housekeeping genes alone can be biased.

A better method is to normalize your data using our qPCR Human Reference cDNA. Because it is prepared from a total RNA pool collected from several different tissues, it provides broad gene coverage, as shown by microarray analysis of the RNA starting material. Lot-to-lot variation is minimal because the RNA source is prepared on an industrial scale. Both high and low abundance genes are well represented allowing preparation of a wide range of serially diluted standards for each qPCR assay.

Ordering information

Please note that as from July 1st, 2017 these products are no longer available from Westburg. They will soon be removed from the website.
Cat no. Description
CL 636690 qPCR Human Reference Total RNA 25 ug
CL 636692 qPCR Human Reference cDNA oligo(dT)-primed 25 rxns
CL 636693 qPCR Human Reference cDNA oligo(dT)-primed 100rxns
CL 639653 qPCR Human Reference cDNA, random-primed 25 rxns
CL 639654 qPCR Human Reference cDNA, random-primed 100 rxns