- Efficiently cleaves TEV site to remove fusion tag
- Use in any buffer suitable for the target protein.
- Carries both GST and His tags for easy protease removal
TEV-Express protease contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly. TEV-Express protease does not require any special buffers for its activity and can be used in a buffer most suitable for the target protein. TEV-Express protease is a 52 kDa protein with both GST and His tags for easy removal using either Amintra NiHIS or Glutathione purification resins.