RTS 100 Wheat Germ Kit

  • Express up to 5 μg protein per reaction in 2.5 hours
  • Synthesis of proteins dependent on eukaryotic chaperones
  • Compatible with non-optimized, pro- and eukaryotic T7 promoter-based vectors as well as linear templates
  • Excellent homogeneity of large proteins (up to 264 kDa expressed)
  • No endotoxins, allows direct use in cell-based assays
  • Upscaling using high yield CECF wheat germ kits
  • Fast parallel synthesis for screening purposes

Efficient cell-free expression in wheat germ extract

The biotechrabbit RTS 100 Wheat Germ Kit is a batch screening system allowing rapid protein expression within 2.5 hours. The kit allows use of a broad range of nonoptimized T7 promoter-based expression vectors from prokaryotic and eukaryotic origin in addition to established cell-free wheat germ vectors or linear templates. Using this approach, multiple constructs — including vectors that have not been optimized for wheat germ — can be screened rapidly for expression and functionality in parallel. The procedure saves precious time otherwise required for template generation. The new batch system yields up to 100 μg protein per ml reaction, which is sufficient for many downstream applications.

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Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the biotechrabbit website to find your local distributor.
Cat no. Description
BR BR1402501 RTS 100 Wheat Germ Kit (24 reactions of 50 µl)