His-Tag Check & Go! Strips

  • Quick yes/no check for presence of his-tagged protein
  • Results in 15 minutes
  • Compatible with cell culture media and lysate
  • Easy to use
  • Cheaper than gel electrophoresis or Western blot
  • Requires only 80┬Ál diluted sample per strip

Rapid check for His-tagged protein

HisTag Check&Go! is a quick competitive immunochromatography test that allows users to verify and monitor successful expression of His-tagged recombinant proteins before undertaking purification, as well as identifying fractions of most interest before performing other more labor intensive techniques such as Western blot or ELISA. The assay provides a qualitative yes/no answer in just 10-15 minutes.

How does it work?

The strips carry a nitrocellulose membrane containing three key components:

  • a Test line of immobilized His-Tag protein
  • a Control line of immobilized ULFA-tagged protein
  • a patch with gold-conjugated antibodies against HisTag and ULFA tag

If your sample contains His-tagged protein this will capture the anti-His antibody, preventing it from binding to the Test line. As a result, the Test line will not be visible or only as a pale red line).

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Expedeon website to find your local distributor.
Cat no. Description
EP 4003-0030 His-Tag Check & Go - 30 strips