Strep-tag protein purification buffers
IBA’s Strep-tag Protein Purification Buffer set is a convenient buffer set for the purification of recombinant Strep-tag fusion proteins on immobilized Strep-Tactin and its regeneration. The Buffer set contains a wash, elution and regeneration buffer. The washing buffer is also separately available.
Strep-tag Regeneration Buffer with HABA gives a clear visualization of the column regeneration process and activity status of the column. The yellow HABA solution turns red upon binding to Strep-Tactin.
Buffer BXT is a Strep-Tactin XT Elution Buffer with Biotin. It contains a Biotin concentration for competitive displacement of purified recombinant Strep-tag®II or Twin-Strep-tag® fusion proteins from immobilized Strep-Tactin®XT.