Prosieve Protrack Dual Color Protein Loading Buffer
- Protects proteins against degradation during heating
- Two tracking dyes: for electrophoresis and blotting
- Contains SDS and DTT
Some proteins are sensitive to pH changes that result from temperature fluctuations during electrophoresis in Tris buffers. The optimized composition of the ProSieve® ProTrack™ Dual Color Protein Loading Buffer protects proteins from heat degradation during the sample preparation step, as well as against pH changes during the SDS-PAGE run.
The loading buffer contains two tracking dyes: blue (bromophenol blue) for tracking the progress of electrophoresis and pink (pyronin Y) for monitoring of protein transfer to the membrane during Western blotting procedures.