Amintra Protein L Resin
- High affinity binding of wide range of mammalian antibody types
- Detects whole Ig classes IgG, IgM, IgA, IgE and IgD
- Detects antibody fragments incl. Fab, scFv and Dab
- Stable and robust
- Re-usable in column format
Protein L binds a wider range of antibody classes and fragments
Compared to the conventional antibody binding proteins A and G, Protein L Agarose Resin offers binding to a wider range of mammalian Immunoglobulin classes and sub-classes. High binding affinity is achieved through a specific interaction of protein L with kappa light chains without interference with the antigen binding site. Thia allows purification of whole IgG, IgM, IgA, IgE and IgD from various species. Besides entire antibody, Protein L can also bind of a wide range of only antibody fragments such as Fab fragments, single-chain variable fragments (scFv), and domain antibodies (Dabs) as lomng as they contain light chains of type kappa I, III or IV in human and kappa in mouse.
Robust antibody purification resin
Amintra Protein L resin carries Protein L immobilized through covalent attachment to agarose beads. This manufacturing process avoids protein loss and provides long-term robustness. Amintra Protein L resin allows batch or column purification of immunoglobulins from cell culture media or biologicial fluids.
Specifications or Protein L Agarose Resin
Supporting matrix: 4% crosslinked agarose resin
Ligand Density: 3,5 mg Protein L/ml resin
Bead size range: 50-150 μm (approx.)
Typical binding capacity: 10 mg Human IgG /ml resin
Chemical stability: Covalent bond
Supplied as a suspension in 20% ethanol.