Ni-NTA Agarose (Sepharose™)

  • Original Ni-NTA Agarose resins: reliable purification
  • High binding capacity for his-tagged proteins
  • Choose economical suspension or convenient prepacked column

Ni-NTA Agarose is the classic resin for purification of 6xHis-tagged proteins by gravity flow chromatography.

Ni-NTA Agarose offers high binding capacity and very low non-specific binding. Ni-NTA Agarose has excellent handling properties for most scales of batch and column purification.

For highest convenience choose pre-packed Ni-NTA Agarose gravity flow columns, available in 1ml and 5ml sizes.

Ni-NTA Agarose resin specifications

  • Binding capacity: up to 50 mg/ml (up to 2500 nmol @ ~20 kDa)
  • Support: Sepharose™ CL-6B
  • Bead structure: Cross-linked, 6% agarose
  • Bead size: 45-165 µm
  • Form (bulk): 50% suspension in 30% ethanol, pre-charged with Ni2+

The Ni-NTA Sepharose™ resin contained in this IBA product is identical to Ni-NTA Agarose manufactured by QIAGEN under a license from Hoffmann-LaRoche Inc.

Sepharose™ is a trademark of GE Healthcare companies.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Finland, Luxembourg, Denmark, Sweden, Norway, Iceland,
If you are not located in these countries please visit the IBA website to find your local distributor.
Cat no. Description
IB 2-3201-010 Ni-NTA Agarose 20 ml
IB 2-3201-025 Ni-NTA Agarose 50 ml
IB 2-3201-100 Ni-NTA Agarose 200 ml
IB 2-3201-500 Ni-NTA Sepharose TM (Agarose) 1000 ml
IB 2-3202-001 Ni-NTA Sepharose (Agarose) gravity flow column 1 ml
IB 2-3202-051 Ni-NTA Sepharose (Agarose) gravity flow column 5 ml
IB 2-9998-001 Empty gravity flow columns (5)