Strep-Tactin XT Resin
- Higher Strep-tag II binding affinity
- Great for High-throughput screening, Batch purification, Denaturating conditions
- More concentrated target protein
- Higher protein yield
Strep-Tactin XT offers higher affinity
The new Strep-Tactin XT has a higher binding affinity to Strep-tag II and is more stable compared to the original Strep-Tactin. This enables new applications such as high-throughput screening, batch purification and denaturing purification.
Enhanced binding of Twin-Strep-tag-mCherry to Strep-Tactin XT compared to Strep-Tactin. A significant loss of mCherry occurred during the washing steps if Strep-Tactin is used for purification. In the case of Strep-Tactin®XT no target protein is lost during the purification procedure. Western blot analysis revealed that remaining impurities were degradation products of mCherry.
Strep-Tactin XT also provides higher protein yields compared to Strep-Tactin®. Together with an optimized elution profile this gives you more concentrated target protein. At the same time Strep-Tactin XT still has all the benefits of Strep-Tactin for protein purification: very high purity (>95%), physiological buffer conditions and mild elution.