Odyssey CLx Western Blot Detection System

  • For quantitative, reproducible Western Blot data analysis
  • Near-infrared (NIR) fluorescence western blot imaging system
  • 2-color fluorescence (700 nm and 800 nm)
  • High sensitivity, low background
  • Also ideal for in vitro & ex vivo imaging probe evaluation

NIR fluorescent western blot imaging: don't compromize data accuracy

Near-infrared (NIR) fluorescent western blot imaging allows you to get better sensitivity without compromising data accuracy.

The Odyssey CLx Western Blot Imaging System is equipped with laser-based illumination and advanced optics: an imager with a wider linear dynamic range compared to chemiluminescence and visible fluorescence.

More consistent, quantitative and reproducible Western Blot results

NIR fluorescence detection doesn’t require chemiluminescent substrates or film. The near-infrared fluorescent signals are stable and reproducible for months, because no enzymes or substrates are used. Direct fluorescent western Blot detection using stable NIR fluorescence signals yields consistent Western Blot results without the variables of enzymatic reactions, like time of exposure, substrate type and availability.

You can even image your Western Blot whenever it suits you or re-image the same blot later and see the same results.

Fluorescent (direct) detection versus ECL (indirect) detection Fluorescent (direct) detection versus ECL (indirect) detection

Complete Western Blot data: see strong and weak Western Blot bands in the same exposure

The Odyssey CLx Western Blot Detection System gives you clear, sharp, reproducible Western Blot bands without blown out blobs. Whereas most Western Blot CCD imagers do not have the capacity to collect all of your data due to signal saturation, with an unprecedented 6 logs of linear dynamic range of the Odyssey CLx, you’ll never lose data because of image saturation.

The Odyssey CLx Western Blot imager captures and analyzes all of your data, without image saturation. The Odyssey CLx Western Blot imager captures and analyzes all of your data, without image saturation.

Multiplexing: detect multiple targets on the same Western Blot

The two infrared fluorescent detection channels (700 nm and 800 nm, respectively) of the Odyssey CLx enables simultaneous detection of multiple proteins on a single blot, without the need for stripping and re-probing. Just use secondary antibodies labeled with spectrally-distinct NIR fluorescent dyes to get more data from your Western Blot. Consistency and reproducibility are improved because you can account for lane-to-lane variation in loading and transfer without stripping and re-probing.

Multiplex to detect two different protein targets in each sample lane. Multiplex to detect two different protein targets in each sample lane.

Odyssey imagers for more applications

The Odyssey CLx can be used for more applications than quantitative and reproducible Western Blot analysis, such as EMSA assays, gel documentation, tissue section imaging, RNAi analysis and more. View the complete list of applications here.

If you need both NIR imaging and chemiluminescence, than find out how our Odyssey FC Western Blot Imaging System can help you get robust and replicable Western Blot data with both methods.

Want accurate & reproducible Western Blot data?

Journals like Science, Nature, Cell, and the Journal of Biological Chemistry are changing minimum content requirements and adopting rigorous Western Blot publishing guidelines.

Do yo also want more accurate and reproducible Western Blot data that is ready for publication? But are you not sure if you're Western Blot workflow meets the requirements needed? Find out more today!

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the LI-COR website to find your local distributor.
Cat no. Description
LI 9140-00 Odyssey CLx Infrared Western Blot Imaging System