Odyssey CLx Western Blot Detection System

  • For quantitative, reproducible Western Blot data analysis
  • Use of direct near-infrared (NIR) fluorescence detection
  • 2-color fluorescence
  • High sensitivity, low background
  • No film, no darkroom, no chemi substrates

NIR imaging of Western Blots with wide linear dynamic range

Near-infrared (NIR) fluorescence detection allows you to get better Western Blot sensitivity without compromising Western Blot data accuracy. The Odyssey CLx Western Blot Detection System is equipped with laser-based illumination and advanced optics: an imager with a wide linear dynamic range that chemiluminescence and visible fluorescence cannot offer.

More consistent and quantitative Western Blot data

NIR fluorescence detection doesn’t require chemiluminescent substrates or film. The near-infrared fluorescent signals are stable and reproducible for months, because no enzymes or substrates are used. Direct detection using stable NIR fluorescence signals yields consistent data without the variables of enzymatic reactions, like time of exposure, substrate type and availability.

You can even image your Western Blot whenever it suits you or re-image the same blot later and see the same results.

Fluorescent (direct) detection versus ECL (indirect) detection Fluorescent (direct) detection versus ECL (indirect) detection

See strong and weak Western Blot bands in the same exposure

The Odyssey CLx Western Blot Detection System gives you clear, sharp, reproducible Western Blot bands without blown out blobs. Whereas most CCD imagers do not have the capacity to collect all of your data due to signal saturation, with an unprecedented 6 logs of linear dynamic range of the Odyssey CLx, you’ll never lose data because of image saturation.

The Odyssey CLx Western Blot imager captures and analyzes all of your data, without image saturation. The Odyssey CLx Western Blot imager captures and analyzes all of your data, without image saturation.

Multiplexing: detect multiple targets on the same Western Blot

The two infrared fluorescent detection channels (700 nm and 800 nm, respectively) of the Odyssey CLx enables simultaneous detection of multiple proteins on a single blot, without the need for stripping and re-probing. Just use secondary antibodies labeled with spectrally-distinct NIR fluorescent dyes to get more data from your Western Blot. Consistency and reproducibility are improved because you can account for lane-to-lane variation in loading and transfer without stripping and re-probing.

Multiplex to detect two different protein targets in each sample lane. Multiplex to detect two different protein targets in each sample lane.

More applications

The Odyssey CLx can be used for more applications than quantitative and reproducible Western Blot analysis, such as EMSA assays, gel documentation, tissue imaging, RNAi analysis and more. View the complete list of applications here.

NIR imaging and chemiluminescence

If you need both NIR imaging and chemiluminescence, than find out how our Odyssey FC Western Blot Imaging System can help you get robust and replicable Western Blot data with both methods.

 
Dowload our Integrated Imaging Solutions brochure for reproducible Western Blots
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  • You can download our  Integrated Imaging Solutions brochure for reproducible Western Blots by filling in the form below.

    If you would like to be contacted for further explanation about Near-InfraRed technology and/or get a demo at your lab, you can also indicate that.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the LI-COR website to find your local distributor.
Cat no. Description
LI 9140-00 Odyssey CLx Infrared Western Blot Imaging System