Odyssey CLx Western Blot Detection System

  • For quantitative, reproducible Western Blot data analysis
  • Use of direct near-infrared (NIR) fluorescence detection
  • 2-color fluorescence
  • High sensitivity, low background
  • No film, no darkroom, no chemi substrates

Direct near-infrared (NIR) fluorescence detection on the Odyssey CLx Western Blot Detection System has become the established standard for quantitaive Western blot analysis. Infrared detection gives you the quantitative Western analysis and wide linear dynamic range that chemiluminescence and visible fluorescence cannot offer.

More consistent, reproducible Western Blot data

Minimize the number of manual steps that can introduce variability to your Western Blot data and increase your Western Blot reproducibility: NIR fluorescence detection doesn’t require chemiluminescent substrates or film. The near-infrared fluorescent signals are stable and reproducible for months, because no enzymes or substrates are used, and so you can image your blots when it’s convenient for you. You can even re-image the same blot later and see the same results. Just place your blot inside the Odyssey CLx system and start collecting data in digital format.

Fluorescent (direct) detection versus ECL (indirect) detection Fluorescent (direct) detection versus ECL (indirect) detection

See strong and weak Western Blot bands in the same exposure

The Odyssey CLx Western Blot Detection System gives you clear, sharp, reproducible Western Blot bands without blown out blobs. Whereas most CCD imagers do not have the capacity to collect all of your data due to signal saturation, with an unprecedented 6 logs of linear dynamic range of the Odyssey CLx, you’ll never lose data because of image saturation.

The Odyssey CLx Western Blot imager captures and analyzes all of your data, without image saturation. The Odyssey CLx Western Blot imager captures and analyzes all of your data, without image saturation.

Multiplexing: detect multiple targets on the same Western Blot

The two infrared fluorescent detection channels (700 nm and 800 nm, respectively) of the Odyssey CLx enables simultaneous detection of multiple proteins on a single blot, without the need for stripping and re-probing. Just use secondary antibodies labeled with spectrally-distinct NIR fluorescent dyes to get more data from your Western Blot. Consistency and reproducibility are improved because you can account for lane-to-lane variation in loading and transfer without stripping and re-probing.

Multiplex to detect two different protein targets in each sample lane. Multiplex to detect two different protein targets in each sample lane.

More applications

The Odyssey CLx can be used for more applications than quantitative and reproducible Western Blot analysis, such as EMSA assays, gel documentation, tissue imaging, RNAi analysis and more. View the complete list of applications here.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the LI-COR website to find your local distributor.
Cat no. Description
LI 9140-00 Odyssey CLx Infrared Western Blot Imaging System