IRDye 680LT Secondary Antibodies

  • Near-infrared dye that is highly soluble in water
  • Most bright and photostable 700 nm near-infrared dye
  • Brightness is comparable to Alexa Fluor® 680
  • Excellent photostability for fluorescence microscopy
  • Low background at near-infrared wavelengths

IRDye 680LT secondary antibodies have been proven the brightest signal and are comparable to Alexa Fluor 680 secondary antibodies. IRDye 680LT is an excellent choice for quantitative Western Blot detection in the 700nm channel and its superb photostability makes IRDye 680LT the best choice for fluorescence microscopy.

IRDye 680LT antibodies are an excellent choice for low abundance targets and can be used for 2-color detection when multiplexed with IRDye 800CW secondary antibodies.

IRDye 680LT are well suited for use on the Odyssey® CLx and Odyssey Fc. IRDye 680LT is available in the following forms: NHS ester, maleimide, carboxylate, secondary antibody conjugates, and in labeling kits.

Comparable fluorescence intensity of IRDye 680LT to Alexa Fluor 680 Comparable fluorescence intensity of IRDye 680LT to Alexa Fluor 680
Comparable photostability of IRDye 680LT to Alexa Fluor 680. Comparable photostability of IRDye 680LT to Alexa Fluor 680.

NOTE:

These antibodies are not recommended when getting up and running on system. Once established near-infrared protocols are optimized with IRDye 680RD, IRDye 680LT can be used to optimize signals in the 700 channel. Dilution range 1:20,000 – 1:40,000. Note: optimization may be required with IRDye 680LT.

Please note that 680LT is not recommended for In-Cell Western, On-Cell Western or small animal imaging. For those applications use IRDye 680RD secondary antibody instead.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the LI-COR website to find your local distributor.
Cat no. Description
LI 925-68020 IRDye 680LT Goat Anti-Mouse IgG (H+L) (0.1mg)
LI 925-68021 IRDye 680LT Goat Anti-Rabbit IgG (H+L) (0.1mg)
LI 925-68022 IRDye 680LT Donkey Anti-Mouse IgG (H+L) (0.1mg)
LI 925-68023 IRDye 680LT Donkey Anti-Rabbit IgG (H+L) (0.1mg)
LI 925-68024 IRDye 680LT Donkey Anti-Goat IgG (H+L) (0.1mg)
LI 925-68028 IRDye680LT Donkey Anti-Chicken IgG (H+L) (0.1mg)
LI 925-68029 IRDye 680LT Goat Anti-Rat IgG (H+L) (0.1mg)
LI 925-68030 IRDye 680LT Donkey Anti-Guinea Pig IgG (H+L) (0.1mg)
LI 925-68031 IRDye 680LT Streptavidin (0.1mg)
LI 925-68032 IRDye 680LT Goat Anti-Human IgG (H+L) (0.1mg)
LI 925-68080 IRDye 680LT Goat anti-Mouse IgM (0.1mg)
LI 926-68020 IRDye 680LT Goat anti-Mouse IgG (0.5 mg)
LI 926-68021 IRDye 680LT Goat anti-Rabbit IgG (0.5 mg)
LI 926-68022 IRDye 680LT Donkey anti-Mouse IgG (0.5 mg)
LI 926-68023 IRDye 680LT Donkey anti-Rabbit IgG (0.5 mg)
LI 926-68024 IRDye 680LT Donkey anti-Goat IgG (0.5 mg)
LI 926-68028 IRDye 680LT Donkey anti-Chicken IgG (0.5mg)
LI 926-68029 IRDye 680LT Goat anti-Rat IgG (0.5 mg)
LI 926-68030 IRDye 680LT Donkey anti-GuineaPig IgG (0.5mg)
LI 926-68031 IRDye 680LT Streptavidin (0.5mg)
LI 926-68032 IRDye 680LT Goat Anti-Human IgG (H+L) (0.5mg)
LI 926-68050 IRDye 680LT Goat Anti-Mouse IgG1 Specific (0.5mg)
LI 926-68051 IRDye 680LT Goat Anti-Mouse IgG2a Specific (0.5mg)
LI 926-68052 IRDye 680LT Goat Anti-Mouse IgG2b Specific (0.5mg)
LI 926-68080 IRDye 680LT Goat anti-Mouse IgM, (0.5mg)