LumiBlue ECL Western Blot Substrates
- Linear response due to long signal duration (6-18 hours)
- High signal intensity
- Range of substrate sensitivities down to 50 fg
- Working solution stable for 5 days (ECL Express)
- Replace other ECL substrates without protocol change
LumiBlue ECL solutions are designed for Western blotting applications with HRP-labeled antibodies. LumiBlue ECL Substrates use patented enhancers to produce bright bands for identification and quantification of proteins on Western Blots.
The LumiBlue ECL range has been designed to match any existing ECL substrate with little to no difference in protocol and have been tuned for a range of antibody dilutions and protein concentrations.
ECL substrate selection guide
|Signal duration||Sensitivity||Comparable to|
|ECL Express||16 hours||Low pg (~20 pg)||ECL Pierce (Thermo) / ECL Start (GE)|
|ECL Pico||6 hours||Low pg (~2 pg)||SuperSignal Pico (Thermo)|
|ECL Extra||12 hours||High fg (~500 fp)||ECL Plus/Prime (Thermo/GE)|
|ECL Extended||18 hours||Mid fg (~150 fg)||SuperSignal Dura (Thermo) / ECL Prime (GE)|
|ECL Extreme||8 hours||Low fg (~50 fg)||SuperSignal Femto (Thermo)/ECL Select (GE)|