LumiBlue ECL Western Blot Substrates

  • Linear response due to long signal duration (6-18 hours)
  • High signal intensity
  • Range of substrate sensitivities down to 50 fg
  • Working solution stable for 5 days (ECL Express)
  • Replace other ECL substrates without protocol change

LumiBlue ECL solutions are designed for Western blotting applications with HRP-labeled antibodies. LumiBlue ECL Substrates use patented enhancers to produce bright bands for identification and quantification of proteins on Western Blots.

The LumiBlue ECL range has been designed to match any existing ECL substrate with little to no difference in protocol and have been tuned for a range of antibody dilutions and protein concentrations.

ECL substrate selection guide

  Signal duration Sensitivity Comparable to
ECL Express 16 hours Low pg (~20 pg) ECL Pierce (Thermo) / ECL Start (GE)
ECL Pico 6 hours Low pg (~2 pg) SuperSignal Pico (Thermo)
ECL Extra 12 hours High fg (~500 fp) ECL Plus/Prime (Thermo/GE)
ECL Extended 18 hours Mid fg (~150 fg) SuperSignal Dura (Thermo) / ECL Prime (GE)
ECL Extreme 8 hours Low fg (~50 fg) SuperSignal Femto (Thermo)/ECL Select (GE)

 

Ordering information

Westburg offers this product in The Netherlands, Belgium, Finland, Luxembourg, Denmark, Sweden, Norway, Iceland,
If you are not located in these countries please visit the Expedeon website to find your local distributor.
Cat no. Description
EP ECLA0250 LumiBlue ECL Extra Substrate (250 ml)
EP ECLA0500 LumiBlue ECL Extra Substrate (500 ml)
EP ECLD0100 LumiBLue ECL Extended Substrate (100 ml)
EP ECLD0200 LumiBLue ECL Extended Substrate (200 ml)
EP ECLM0100 LumiBlue ECL Extreme Substrate (100 ml)
EP ECLM0200 LumiBlue ECL Extreme Substrate (200 ml)
EP ECLP0100 LumiBlue ECL Pico Substrate (100 ml)
EP ECLP0250 LumiBlue ECL Pico Substrate (250 ml)
EP ECLP0500 LumiBlue ECL Pico Substrate (500 ml)
EP ECLS0100 LumiBlue ECL Express Substrate (100 ml)
EP ECLS0250 LumiBlue ECL Express Substrate (250 ml)
EP ECLS0500 LumiBlue ECL Express Substrate (500 ml)