WesternSure ECL Stripping Buffer
- Effectively strips antibodies from Western blots
- Mild buffer leaves target proteins on blot intact
- One buffer for PVDF and nitrocellulose membranes
WesternSure ECL Stripping Buffer is ideal for stripping and reprobing chemiluminescent Western blots.
WesternSure ECL Stripping Buffer is effective for reprobing with different antibodies
Original: A Western blot with EGF-stimulated A431 lysate (10 and 5 µg total protein) was probed with mouse anti-ERK2 followed by detection with WesternSure HRP Goat anti-Mouse and WesternSure PREMIUM Chemiluminescent Substrate. The blot was exposed to film for 20 sec.
Stripped: The blot was then stripped with WesternSure ECL Stripping Buffer, followed by detection with WesternSure PREMIUM, and exposed to film for 10 min.
Reprobed: The blot was re-blocked and reprobed with Beta-Actin Monoclonal followed by detection with HRP Goat anti-Mouse and WesternSure PREMIUM. The blot was exposed to film for 20 sec.
Stripping Buffer for antibody optimization
WesternSure ECL Stripping Buffer is not recommended for loading amounts over 30 µg. It will demonstrate decreased performance when used at 2X or 5X concentration and heating the solution will not increase the stripping efficiency.