REVERT Total Protein Stain
- Accurate Western blot normalization: linear range of 1-60 µg
- Fast total protein stain (<10 minutes)
- Compatible with PVDF and nitrocellulose Western blots
- More reliable than housekeeping proteins
- For near-infrared (NIR) fluorescent detection
Accurate Western blot normalization
REVERT is a total protein stain that binds to any protein in your samples after transfer from gel to the blot membrane. The stain provides a linear signal over a broad range of protein loading and does not interfere with subsequent Western blot detection.
REVERT total protein stain is more reliable than housekeeping proteins, due to its broad binding to any protein and wide linear range detection of of 1-60 µg protein.
REVERT is ideal for accurate Western blot normalization.
Compatible with PVDF and nitrocellulose Western blots
You can easily integrate REVERT Total Protein Stain in your existing Western blotting workflow. You don't need any additional reagents or special gels. Both PVDF and nitrocellulose blots are stained within 10 minutes.
More accurate normalization than housekeeping proteins
Normalize your quantitative Western blots more accurately with REVERT by correcting for well-to-well variation in sample loading and transfer. REVERT Total Protein Stain gives a linear signal over a wider range of cell lysate concentration than commonly used housekeeping proteins.
Which product to choose?
The REVERT stain is detected in the 700 nm channel of the Odyssey. Therefore, the choice of REVERT product depends on whether you want to detect one or two targets on the Western blot.
In this case you can detect your target in the 800 nm channel, so the stain can be left on the blot.
Order cat. no. LI 926-11015 REVERT Total Protein Stain and Wash Solution Kit. It contains 100 ml REVERT Total Protein Stain and 200 ml Wash solution.
Here you will need to remove the stain before immunodetection to allow detection of the second target in the 700 nm channel.
Order cat. no. LI 926-11010 REVERT Total Protein Stain Kit. It contains 100 ml REVERT Total Protein Stain, 200 ml Wash solution and 200 ml Reversal solution