Klenow Fragment is a mesophilic DNA polymerase derived from the E.coli Polymerase I DNA-dependent repair enzyme. The enzyme exhibits DNA synthesis and proofreading (3′→5′) nuclease activities, and, in the absence of the holoenzyme’s (5′→3′) nuclease domain, displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene.
Source of Klenow Fragment
A recombinant E. coli strain carrying the Klenow Fragment gene.
Concentration of Klenow fragment: 5,000 U/mL
- Purity (SDS-PAGE) >99%
- Specific Activity 5,000 U/mg
- SS Exonuclease Functional
- DS Exonuclease Functional
- DS Endonuclease 50 U = no conversion
- E.coli DNA Contamination 50 U < 10 copies
Bulk Klenow Fragment for NGS applications
Need larger quatities of Klenow polymerase for your NGS protocols? Contact us for further information and a price quote.