Klenow Fragment

Klenow Fragment is a mesophilic DNA polymerase derived from the E.coli Polymerase I DNA-dependent repair enzyme. The enzyme exhibits DNA synthesis and proofreading (3′→5′) nuclease activities, and, in the absence of the holoenzyme’s (5′→3′) nuclease domain, displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene.

Source of Klenow Fragment

A recombinant E. coli strain carrying the Klenow Fragment gene.


Concentration of Klenow fragment: 5,000 U/mL

QC tests:

  • Purity (SDS-PAGE) >99%
  • Specific Activity 5,000 U/mg
  • SS Exonuclease Functional
  • DS Exonuclease Functional
  • DS Endonuclease 50 U = no conversion
  • E.coli DNA Contamination 50 U < 10 copies

Bulk Klenow Fragment for NGS applications

Need larger quatities of Klenow polymerase for your NGS protocols? Contact us for further information and a price quote.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the MB Enzymes website to find your local distributor.
Cat no. Description
EZ P7060L Klenow Fragment 2500 units (5,000 U/ml)